DNFB-induced contact hypersensitivity model

A well-established murine model of delayed-type hypersensitivity (DTH)that is used in our department  is characterized with respect to liposome accumulation at a site of inflammation. Here, mice were sensitized by painting the abdominal region with a solution of 2,4-dinitrofluorobenzene (DNFB) and inflammation was induced 5 days later by challenging the ear with a dilute solution of DNFB. The inflammatory response can be readily monitored by measuring ear thickness (edema) and radiolabeled leukocyte infiltration.