Immunization-induced EBA

Experimental EBA can also be induced in susceptible mouse strains by either single (J Invest Dermatol, 2010) or repetative (J Immunol, 2006) immunization with an immunodominant fragment located within type VII collagen NC1 domain. Disease induction requires:

The following therapeutic interventions lead to disease improvement:



Antibody transfer model of EBA

An antibody-transfer of epidermolysis bullosa acquisita (EBA) was originally developed in 2005 (J Clin Invest, 2005). The disease is induced by repetetive injections of rabbit anti-mouse type VII antibodies into susceptible adult mouse strains. Within 2-4 days skin lesions develop. In addition to skin lesions, tissue injury is also induced throughout the entire gut (J Pathol, 2011).
Overall, this model is ideally suited to investigate autoantibody-induced tissue injury in vivo. Induction of clinical skin lesion depends on: